The Synergistic effect of Nanoparticles (Ag: Se) prepared by Plasma Jet Combined with Plantago lanceolate on Parasitic Leishmania donivani
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Abstract
Visceral leishmaniasis (VL) is a chronic infection caused by protozoa. Drug-resistant cases require an urgent solution to drug resistance and a therapeutic improvement strategy. Nanoparticles (NPs) containing synthetic plant extract exhibit antileishmanial potential as therapeutic agents. This study aims to investigate the synergistic antileishmanial effect of silver: selenium (Ag: Se) core-shell NPs synthesized using the plasma jet technique in combination with Plantago lanceolata leaf extract against Leishmania donovani. The Ag:Se NPs were synthesised and applied at two concentrations, 125 and 250 µg/mL, to assess their potential activity in vitro. The control treatment was miltefosine, an anti-leishmaniasis medication. P. lanceolata extract at concentrations of 125 and 250 µg/mL was used to produce Ag NPs as a core and add selenium as a shell. The generated Ag:Se NPs were characterized using UV-Vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). The anti-leishmanial activity of Ag:Se NPs was evaluated. Ag:Se NPs were assessed at 125 and 250 μg/mL concentrations, with 25%, 50%, and 100% serial dilutions over 1, 24, and 48 h incubation periods. After 48 hours, the percentage of inhibition for Ag:Se NPs (125 and 250 μg/mL) combined with the extract was 48.6% and 59.6%, respectively. Thus, it may be inferred from these results that the growth inhibition rate increases with the length of incubation of the parasite. Antileishmanial activity was improved in the case of Ag:Se NPs medicines designed using a plasma jet.
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© 2023 The Author(s). Published by the College of Science, University of Baghdad. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License.
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